PMB 185: Techniques in Light Microscopy
Denise Schichnes Ph.D.
and
Steve Ruzin, Ph.D. Emeritus
The course is a detailed overview of the practice of light microscopy as applied to scientific investigation. The emphasis of the course is on the use of the light microscope by biological scientists, however students of other disciplines are welcome. Topics covered are optical microscope theory, microscope components and mechanics, and all modern optical techniques of transmitted and fluorescence microscopy. Lectures and hands-on labs cover basic optics followed by Brightfield through Super-Resolution microscopy.
This class uses the following text:
Ruzin SE, 2025. Techniques in Light Microscopy. Oxford University Press.