The Biological Imaging Facility is a core microscope imaging facility that specializes in widefield fluorescence, laser scanning confocal, spinning disk confocal, TIRF, and super-resolution microscopy (Lattice SIM, PALM, STORM), as well as traditional plant & animal microtechnique, histology, and cryotomy.

IOM112024 malaria parasite

Murine hepatocytes (liver cells) that are infected with malaria (Plasmodium berghei). A parasite membrane protein is stained in green, the nucleus of the cell in blue and the cell cytoskeleton in red. This image was collected using BIF’s Zeiss 880 confocal, and processed using Imaris (Bitplane). Scale is 5µm.

Helene Borrmann of the Rijo-Ferreira Lab

The Rausser College of Natural Resource’s Biological Imaging Facility functions as an instructional and research laboratory for all aspects of modern light microscopy, including confocal and super-resolution microscopy, image processing and analysis, and most microscopical techniques for developmental and cell biology. Computer image processing and analysis is taught on an individual basis. Microscopy is taught on two levels:

  • Individually as needed
  • PMB185 Techniques in Light Microscopy (this class has been discontinued as of Fall 2024)

In addition, the Facility offers a one-week workshop in Plant & Animal Microtechnique designed to train the student in modern and classical methods in making microscope slide preparations. Please contact us for more details on our next workshop.

There are four core laboratories on campus that offer expertise, instruction, and instrumentation in microscopy for research.

  • The CNR Biological Imaging Facility (This lab): widefield, confocal, and super-resolution epifluorescence microscopy, live-cell imaging, microtechnique, training in digital image processing and analysis. Located in 381 Koshland Hall
  • Molecular Imaging Center: Two-photon, SPIM, confocal microscopy, electrophysiology, live-cell imaging, and FLIM. Located in Weill, Li Ka Shing, and Barker Halls.
  • Electron Microscope Laboratory: Transmission and scanning electron microscopy. Located in Barker Hall.
  • CIRM/QB3 Shared Stem Cell Facility: Available for the study of stem cells. Located in Stanley Hall
  • The Golub Microscope Collection: Web site dedicated to Dr. Orville J. Golub’s collection of antique microscopes from the 17th–20th Centuries. The collection is located in VLSB. Dr. Steve Ruzin is the Curator.