The Biological Imaging Facility is a core microscope imaging facility that specializes in widefield fluorescence, laser scanning confocal, spinning disk confocal, TIRF, and super-resolution microscopy (Lattice SIM, PALM, STORM), as well as traditional plant & animal microtechnique, histology, and cryotomy.
Vegetative hyphae of Streptomyces coelicolor A3(2) were grown positioned between a glass coverslip and an ISP2-Agar pad. Notably, this particular strain lacks cvnA8, leading to the constitutive activation of the cvn8 operon. To facilitate the real-time monitoring of cvn8 activity, a fluorescent reporter expressing eGFP was constructed. The utilization of this fluorescent reporter allows for precise visualization and quantification of cvn8 operon activation within the vegetative hyphae. Images were collected on the Biological Imaging Facility Elyra7 Lattice SIM superresolution microscope. Inset shows an enlarged view of cells from this image, note the puncta are about 100nm in diameter. Scale bar in insert is 500nm.
Kilian Dekoninck of the Traxler Lab
- Individually as needed
- PMB185 Techniques in Light Microscopy.
- If you wish to be trained, please complete this Google Form, then contact Denise Schichnes. Once you have completed this training form and are trained, you will be authorized to reserve instrument time and enter the BIF.
- If you are already trained feel free to reserve instrument time yourself. Contact us for the Calendar signup credentials.
There are four core laboratories on campus that offer expertise, instruction, and instrumentation in microscopy for research.
- The CNR Biological Imaging Facility (This lab): widefield, confocal, and super-resolution epifluorescence microscopy, live-cell imaging, microtechnique, training in digital image processing and analysis. Located in 381 Koshland Hall
- Molecular Imaging Center: Two-photon, SPIM, confocal microscopy, electrophysiology, live-cell imaging, and FLIM. Located in Weill, Li Ka Shing, and Barker Halls.
- Electron Microscope Laboratory: Transmission and scanning electron microscopy. Located in Barker Hall.
- CIRM/QB3 Shared Stem Cell Facility: Available for the study of stem cells. Located in Stanley Hall
- The Golub Microscope Collection: Web site dedicated to Dr. Orville J. Golub’s collection of antique microscopes from the 17th–20th Centuries. The collection is located in VLSB. Dr. Steve Ruzin is the Curator.