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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution, Spinning Disk, and Super-Resolution fluorescence microscopy (SIM, PALM, TIRF).

Past IOMs
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The Shapira Lab investigates the ecology and host-microbe interactions of commensal microbes in the gut of the nematode C. elegans. Commensal strains from the genus Enterobacter isolated from the natural C. elegans gut environment were engineered to express different fluorescent reporters to examine colonization patterns and processes. For this image, an adult worm is seen with intestinal colonization of an Enterobacter strain expressing GFP and the same strain expressing tdTomato - dominance of just the GFP population within the intestine indicates that gut colonization of native commensals may be a rare event driven by stochastic behaviour. Note the near-equal presence of both strains in the pharynx in the head of the worm, similar to the 1:1 co-culture the worms were grown in. The fluorescence on either side of the intestine is due to autofluorescence from the worm itself and neither bacterial strain.

Image contributed by Alex Crits-Christoph of the Shapira lab

Thu, Dec 1, 2016