at the University of California, Berkeley
There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.
The CNR Biological Imaging Facility (This lab): Confocal, deconvolution, and widefield fluorescence microscopy, live-cell imaging, microtechnique, training in digital image processing and analysis. Located in 381 Koshland Hall
Molecular Imaging Center: Multi-photon laser scanning confocal microscopy, electrophysiology, live-cell imaging. Located in LSA and LKS.
This page gives a general overview of many of the research cores on the Berkeley campus.
The Golub Microscope Collection: Web site dedicated to Dr. Orville J. Golub's collection of antique microscopes from the 17th–20th Centuries. The collection is located in VLSB
The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution, Spinning Disk, and Super-Resolution fluorescence microscopy
(SIM, PALM, TIRF).
The ParB protein binds to sequences adjacent to the origin of replication; therefore, a fluorescent fusion to this protein makes it possible to follow the dynamics of the bacterial chromosome during the cell cycle. In short cells (early in the cell cycle), the Agrobacterium tumefaciens ParB (fused to mCherry) is visible as a distinct focus at the old cell pole. As cells grow, two foci are visible indicating the chromosome has been replicated. One of the foci migrates across the cell to the other pole to ensure that each sibling cell has a copy of the chromosome. This image was taken on the Zeiss AxioObserver. Z1 in the BIF.
Image contributed by Jose Sebastian Robalino Espinosa and John Zupan from the Zambryski Lab.