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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution, Spinning Disk, and Super-Resolution fluorescence microscopy
(SIM, PALM, TIRF).

Past IOMs

2006–2018

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Golovinomyces orontii (powdery mildew fungus) infection of Arabidopsis thaliana leaf. After infection, the G. orontii conidium (C) germinated and grew hyphae on the leaf surface. Infected leaf was stained with DAPI (nucleic acid stain) to quantify DNA content of A. thaliana mesophyll cells underlying the fungal feeding structure (haustorium, H) embedded in the leaf epidermis. To determine the DNA content of mesophyll nuclei, fluorescence intensity sum was measured (large gray spheres) and normalized to guard cell nuclei (small grey spheres around stomata opening) as they have a 2C DNA content.  Z-stack was taken with the Zeiss LSM710 and fluorescence intensity sum was measured with Spots application in Imaris v9.  

Image contributed by the Amanda McRae; Ph.D. Candidate, Wildermuth Lab.

Fri, Aug 30, 2019