CNR Biological Imaging Facility, The University of California at Berkeley

Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

The CNR Biological Imaging Facility (This lab): Confocal, deconvolution, and widefield fluorescence microscopy, live-cell imaging, microtechnique, training in digital image processing and analysis. Located in 381 Koshland Hall
Molecular Imaging Center: Multi-photon laser scanning confocal microscopy, electrophysiology, live-cell imaging. Located in LSA and LKS.
Electron Microscopy Laboratory: Transmission and scanning electron microscopy. Located in Giannini Hall.
CIRM/QB3 Shared Stem Cell Facility: Available for the study of stem cells. Located in Stanley Hall
The Golub Microscope Collection: Web site dedicated to Dr. Orville J. Golub's collection of antique microscopes from the 17th–20th Centuries. The collection is located in VLSB

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution, Spinning Disk, and Super-Resolution fluorescence microscopy (SIM, PALM, TIRF).

Past IOMs

The image is of a Pseudomonas syringae B728a strain that has a knock-out in the fleQ gene which is responsible for regulating the flagellar genes. This Pseudomonas strain also harbors a gfp reporter gene fused to the syfA promoter. The bacteria were adhered to 1µm fluorescent microspheres and co-inoculated onto a filter. After a specific time the distance of bacterial migration was determined (red lines). The image was taken on the Zeiss AxioImager M2 epifluorescence microscope using the GFP filter and 100x magnification. Measurements was done using iVision software.

Image contributed by Monica N Hernandez of the Lindow lab.

Microscopy Facilities at the University of California, Berkeley

Microscopy Facilities
at the University of California, Berkeley