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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Live-Cell Widefield, Confocal (including FCS),
Spinning Disk, and Super-Resolution fluorescence microscopy

Past IOMs


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COVID-19 Phase 3 Policy

In order to accommodate more Users in the Facility, we have transitioned to Phase 3.

This means that we will allow up to three (3) users in the BIF at any one time.

We also will be doing Training for New Users.

Before Resuming Work at the BIF, all users must:

1. Read and follow this document.

2. Fill out the BIF COVID USE FORM:

3. View the disinfecting video here for disinfecting BEFORE (to protect you) and AFTER (to protect the next user).

4. New Users: This page explains our Phase 3 Training procedure.

5. Know how to Start Zoom and Join a Zoom Meeting. Do not ask for training or help unless you are capable of doing this.

Once you have completed the BIF Covid Use Form and are approved you will be authorized to enter the BIF.

In order for us to maintain our Density Budget of three (3) people, Denise will arrange ALL scheduling. Please email her and she will insert you in the BIF Covid Phase 3 Calendar.

There can be NO DROP-INS. You must be entered by Denise on the reservation calendar. Note also, while you are working in the BIF you must also follow your lab Density Budget.

Use charges will be based on your calendar reservation. We will NOT delete reservations <24h prior to your start time. So make your reservations carefully.

Please visit the Vice Chancellor for Research website for guidance on Planning for Research Continuity

Colocalization Analysis by HuygensPro. Huygens can generate colocalization coefficients from 2D and 3D datasets from any instrument in the BIF. Analysis is based on either of three threshold estimations, including the Costes method. Here we see the colocalization of fluorescence in two channels (represented as "Red" and "Green") that express a Pierson's Coefficient of 0.676. HuygensPro has created a Surface model of the colocalized voxels of this SIM dataset superimposed over the MIP of the volume. Colocalization is only one of several computational processes that BIF's HuygensPro can perform on your data. Included is Particle Analysis, Deconvolution, Stitching, and Channel alignment.

Tue, Nov 10, 2020