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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

This is an image of in vitro differentiated brown adipocyte in culture, labeled for expression of Rdh10 protein (retinol dehydrogenase 10, one of the major enzyme involved in cellular Vitamin A metabolism, and also has a role in lipid droplet formation). This is a composite image taken using the Zeiss AxioObserver Z1 epifluorescent microscope with 100x objective lens at the Biological Imaging Facility. AlexaFluor594 fluoresce labeled antibody against Rdh10 protein was used to visualize Rdh10 protein in cells (in red), nuclear DAPI stain was in blue, and a bright field image reveals multilocular lipid droplets, a hallmark of fully differentiated brown adipocytes. In the filed of view, cells that do not contain lipid droplets appear not to express Rdh10 protein.

Image contributed by Marta Vuckovic of the Napoli lab, NST

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution and Super-Resolution fluorescence microscopy (SIM, PALM, TIRF).

Past IOMs
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The Zeiss Elyra SIM microscope is up and running.

We have also purchased a major upgrade to our Live-Cell AxioObserver system. The scope will have laser-based autofocus, an environmental chamber, and a LED fluorescence excitation light source. We hope to have the scope upgraded by late Oct.

Tue, Oct 7, 2014