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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

Chlamydomonas reinhardtii is a unicellular green alga that is not usually easy to image under a microscope because of flagellar-mediated cell movement. For this image, however, we stabilized a single WT cell using a CellASIC microfluidics system and subsequently imaged that cell over a 5-minute time period using 3D SIM. The time-lapse image of chlorophyll autofluorescence shows thylakoid membrane movement in the chloroplast. The movie shows that some parts of thylakoid membrane system change position dynamically. The precise function of such membrane dynamics is unknown, but thylakoid membrane protein reorganization might be involved. Images were acquired on the Zeiss Elyra PS.1 Structured Illumination Microscope in the BIF. (Click image to see movie).

Image contributed by Masakazu Iwai of the Niyogi lab PMB

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution and Super-Resolution fluorescence microscopy (SIM, PALM, TIRF).

Past IOMs
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Tue, May 5, 2015