CNR Biological Imaging Facility, The University of California at Berkeley

Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

The CNR Biological Imaging Facility (This lab): Confocal, deconvolution, and widefield fluorescence microscopy, live-cell imaging, microtechnique, training in digital image processing and analysis. Located in 381 Koshland Hall
Molecular Imaging Center: Multi-photon laser scanning confocal microscopy, electrophysiology, live-cell imaging. Located in LSA and LKS.
Electron Microscopy Laboratory: Transmission and scanning electron microscopy. Located in Giannini Hall.
CIRM/QB3 Shared Stem Cell Facility: Available for the study of stem cells. Located in Stanley Hall
Core Facilities on the Berkeley campus.
The Golub Microscope Collection: Web site dedicated to Dr. Orville J. Golub's collection of antique microscopes from the 17th–20th Centuries. The collection is located in VLSB

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Live-Cell Widefield, Confocal (including FCS),
Spinning Disk, and Super-Resolution fluorescence microscopy
(SIM, PALM, TIRF).

Past IOMs

2006–2019

NEW COVID-19 Policy

In order to accommodate more Users in the Facility, we have received VCR Approval to allow users into BIF for the duration of Phase 2.

In order for any VCR-approved researcher to gain access to the BIF you must follow certain rules and guidelines.

Before Resuming Work at the BIF, all users must:

1. Read and follow this document.

2. Fill out the BIF COVID USE FORM:

3. View the disinfecting video here for disinfecting BEFORE (to protect you) and AFTER (to protect the next user).

Once you have completed the BIF Covid Use Form and are approved you will be authorized to enter the BIF.

In order for us to maintain our Density Budget of two (2) people, Denise will arrange ALL scheduling. Please email her and she will insert you in the BIF Covid Phase 2.2 Calendar.

There can be NO DROP-INS. You must be entered by Denise on the reservation calendar. Note also, while you are working in the BIF you must also follow your lab Density Budget.

Use charges will be based on your calendar reservation. We will NOT delete reservations <24h prior to your start time. So make your reservations carefully.

Due to the requirement for Social Distancing, there will be NO New User trainings during Phase 2.

Please visit the Vice Chancellor for Research website for guidance on Planning for Research Continuity

Different types of myofibers were stained with MYH antibodies: type I (blue, stained with MYH7, oxidative fibers consume fatty acid), type IIA (green, stained with MYH2, intermediate fibers consume both glucose and fat), and type IIB (red, stained with MYH4, glycolytic fibers consume glucose). Samples were collected from gastrocnemius muscle in Rdh10-whole body knockout mice fed on a high-fat diet. In this project, we detected more type I fibers in the male KO mice as a compensation to the impaired mitochondria function. Images were taken on the AxioImager M1 using the Hamamatsu digital camera in BIF.

Image contributed by Yaxin Zhao from Napoli lab

Microscopy Facilities at the University of California, Berkeley

Microscopy Facilities
at the University of California, Berkeley