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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

This picture, taken on the LSM710 confocal microscope in the BIF using the 20X objective, is a 150 micron thick retinal slice from a mouse that was intravitreally injected with adeno associated virus variant ShH10-Y445F carrying the RGECO transgene under a ubiquitous promoter. Red is RGECO, a red shifted genetically encoded calcium indicator. Green is antibody staining against glutamine synthetase, a Müller cell marker (the primary glial cell of the retina). Blue is DAPI.The ShH10-Y445F AAV variant selectively infects Müller cells, demonstrated by co-localization of RGECO (red) with glutamine synthetase (green).

Imaged by Cameron Baker from the Flannery Lab

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution and Super-Resolution fluorescence microscopy (SIM, PALM, TIRF).

Past IOMs
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The Zeiss Elyra SIM microscope is up and running.

The Live-Cell AxioObserver Z1 system has been updated. It now has an environmental chamber and Zeiss' Definite Focus. It is ready for use.

Tue, Jan 6, 2015