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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution, Spinning Disk, and Super-Resolution fluorescence microscopy (SIM, PALM, TIRF).

Past IOMs
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The image is a grape pathogen Xylella fastidiosa TEM1 that has a mutation in rpfF synthase gene regulating production of a diffusible signaling factor (DSF). This Xf mutant is unable to produce its own DSFs but can sense DSF signals from other bacteria as well as externally added fatty acid molecules. It also harbors an alkaline phosphatase (phoA) reporter gene fused to the DSF-regulated hxfA promoter. The reporter was activated by addition of 3 mM C16-cis fatty acid (2(Z)-hexadecenoic acid). Phosphatase activity was detected by the ELF® 97 Endogenous Phosphatase substrate that upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. Xf bacteria are stained with DAPI.The image was taken on the Zeiss AxioImager M2 epifluorescence microscope using DAPI filter and 100x 1.4NA objective.

Image contributed by Elena Antonova of the Lindow lab

Tue, Oct 4, 2016