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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution, Spinning Disk, and Super-Resolution fluorescence microscopy
(SIM, PALM, TIRF).

Past IOMs
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This is an image of a transversely cut hind-leg skeletal muscle stained with Lillie’s Modified Trichrome. The nuclei are stained dark purple, cytoplasm in red and collagenous connective tissue in blue-green. In this image a muscle spindle with intrafusal muscle fibers, surrounded by a connective tissue capsule, and a vein can also be seen in the upper right hand side of the image. The image shows muscle pathology, specifically fibrosis, indicated by muscle fiber separation, collagen infiltration and a thick endomycium surrounding the fibers. Fibrosis is the result of the deletion of Reinol Dehydrogenase 10 (Rdh10) involved in the first step of Vitamin A metabolism and a high fat diet. This image was produced with the help of Dr. Schichnes and Dr. Ruzin and taken with the AxioImagerM1 microscope in the Biological Imaging Facility.

Image contributed by Milena Tintcheva from the Napoli Lab

Tue, Apr 4, 2017