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Microscopy Facilities
at the University of California, Berkeley

There are three core laboratories on campus that offer expertise, instruction, and instrumentation in optical microscopy for research.

Many rod-shaped bacteria, including pathogens such as Brucella and Mycobacterium, or Agrobacterium grow by adding new peptidoglycan (PG) to their cell poles. This is a 3D reconstruction from SIM images of Atu0845-superfolding GFP expressed in Agrobacterium. Atu0845, a periplasmic L,D-transpeptidase which crosslinks adjacent PG strands, is located at the growth pole, but is also weakly visible at the old pole.  Cell polarity is determined with the lipophilic dye FM4-64 which preferentially labels the old pole more intensely than the growth pole. (Click image to play the 3D movie). Images were acquired on the Zeiss Elyra PS.1 Structured Illumination Microscope in the BIF.

Image contributed by Romain Grangeon and John Zupan of the Zambryski lab PMB

The Biological Imaging Facility is a core microscope imaging lab at the University of California, Berkeley.
The BIF specializes in Widefield, Confocal, Deconvolution and Super-Resolution fluorescence microscopy (SIM, PALM, TIRF).

Past IOMs
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Mon, Apr 6, 2015