Technologies available in the
BIOLOGICAL IMAGING FACILITY
UC Berkeley
1. Microtechnique
- Paraffin sectioning for general light microscopy and in situ hybridization using Microwaves. We use the Microwave Research and Applications, Inc BP-111-RS tissue processor. Here are some samples.
- Plastic (methacrylate) sectioning for general light microscopy, immunolocalization and in situ hybridization.
- Cryomicrotomy for immunolocalization and in situ hybridization.
- The Facility offers each June a 5-day intensive Workshop in Plant & Animal Microtechnique.
2. Light microscopy
- Zeiss 510 Meta UV/Vis Confocal Laser Scanning Microscope
- Applied Precision DeltaVision Spectris (DV4) deconvolution microscope.
- Intel Mac Quad Core 3GHz workstation with Bitplane Imaris and SVI Huygens
- Macintosh Quad Core G5 computer for image manipulation and processing using iVision.
- Zeiss Axiophot:
- Bright field, Dark field, Phase, DIC.
- Epifluorescence illumination
- High resolution digital CCD camera (QImaging) for color image capture
- Zeiss AxioImager M1 fluorescence microscope for quantitative fluorescence.
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- Bright field, Dark field, Phase, DIC.
- Epifluorescence illumination
- High resolution digital CCD camera (Photometrics Quantix) for 12-bit image capture
- Zeiss AxioObserver inverted fluorescence microscope for live-cell imaging
Bright field, Dark field, Phase, DIC, PlasDIC for plastic plates
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- Epifluorescence illumination
- High resolution digital CCD camera (Hamamatsu EMCCD 9100-13) for 16-bit image capture
- iVision for scripting long-term experiments
- Zeiss Axiovert for plates
- Bright field, phase, DIC.
- Epifluorescence
- Computer color image capture using Optronics 750 color CCD camera
- Zeiss SV11 stereo microscope
- Zeiss Lumar Epifluorescence stereo microscope for low magnification fluorescence imaging
- Ruzin offers a semester course "Techniques in Light Microscopy".
3. Laser Microdissection
- Leica LMD UV laser microdissection microscope.
4. Low Light Fluorescence and Ratio Imaging
- Low light gray scale fluorescence detection using a Photometrics Quantix high-resolution CCD digital camera
- Automated ratio imaging using iVision
5. Computer image processing and analysis
- Color digital image capture
- Color printing: Photo quality Epson Color Stylus Pro 4000, Tektronix 740 color laser printer
- Image manipulation and enhancement using Photoshop, iVision, and Bitplane Imaris.
- Deconvolution of digital image series using SVI Huygens and API SoftWoRx.
- Morphometrics, particle counting, particle tracking, numeric analysis, Fourier transforms (Imaris)
- AIT data archiving
Revised Wed, Sep 3, 2008