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Technologies available in the
UC Berkeley

1. Microtechnique
  1. Paraffin sectioning for general light microscopy and in situ hybridization using Microwaves. We use the Microwave Research and Applications, Inc BP-111-RS tissue processor. Here are some samples.
  2. Plastic (methacrylate) sectioning for general light microscopy, immunolocalization and in situ hybridization.
  3. Cryomicrotomy for immunolocalization and in situ hybridization.
2. Light microscopy
  1. Zeiss Elyra PS.1 super-resolution SIM/PALM scope.
  2. Zeiss LSM 710 confocal laser scanning microscope.
  3. Leica/Yokagawa Spinning Disk confocal microscope
  4. Applied Precision DeltaVision Elite deconvolution microscope.
  5. Bitplane Imaris for 3D imaging
  6. SVI HuygensPro for deconvolution and colocalization analysis
  7. iVision for 2D image manipulation, processing, and microscope control.
  8. Zeiss AxioImager M2:
    1. Bright field, Dark field, Phase, DIC.
    2. Epifluorescence illumination
    3. High resolution quantitative digital CCD camera (QImaging iClick) for 12-bit image capture
  9. Zeiss AxioImager M1 fluorescence microscope for quantitative fluorescence.
    1. Bright field, Dark field, Phase, DIC.
    2. Epifluorescence illumination
    3. High resolution digital CCD camera (Hamamatsu Orca 03) for 12-bit image capture
  10. Zeiss AxioObserver Z1 inverted fluorescence microscope for live-cell imaging for Bright field, Dark field, Phase, DIC, PlasDIC for plastic plates, Epifluorescence illumination
    1. High resolution digital CCD camera (Hamamatsu EMCCD 9100-13) for 16-bit image capture'
    2. iVision for scripting long-term experiments
  11. Zeiss Axiovert for plates
    1. Bright field, phase, DIC.
    2. Epifluorescence
  12. Zeiss SV11 stereo microscope
  13. Zeiss Stereo Lumar Epifluorescence stereo microscope for low magnification fluorescence imagin
  14. Ruzin offers a semester course "Techniques in Light Microscopy".
  • 3. Low Light Fluorescence for Live-Cell imaging (AxioObserver Z1)

    1. Low light gray scale fluorescence detection using a QImaging Retiga SRV low-light CCD digital camera
    2. Automated timelapse fluorescence imaging using iVision
    3. Full environmental control
    4. Full point-visiting stage control
    4. Computer image processing and analysis
    1. Image manipulation and enhancement using Photoshop, iVision, HuygensPro, and Imaris.
    2. Deconvolution using SVI HuygensPro.
    3. Morphometrics, particle counting, particle tracking, numeric analysis (Imaris)
    4. Colocalization, Object Analysis, and Stitching using HuygensPro
    5. Data backup and archiving

    Revised Wed, Jul 25, 2018