1. Paraffin sectioning for general light microscopy and in situ hybridization using Microwaves. We use the Microwave Research and Applications, Inc BP-111-RS tissue processor. Here are some samples.
2. Plastic (methacrylate) sectioning for general light microscopy, immunolocalization and in situ hybridization.
3. Cryomicrotomy for immunolocalization and in situ hybridization.
4. The Facility offers each June a 5-day intensive Workshop in Plant & Animal Microtechnique.

1. Zeiss 510 Meta UV/Vis Confocal Laser Scanning Microscope
   
2. Applied Precision DeltaVision Spectris (DV4) deconvolution microscope.
   
3. Intel Mac Quad Core 3GHz workstation with Bitplane Imaris and SVI Huygens
   
4. Macintosh Quad Core G5 computer for image manipulation and processing using iVision.
   
5. Zeiss Axiophot:
   • Bright field, Dark field, Phase, DIC.
   • Epifluorescence illumination
   • High resolution digital CCD camera (QImaging) for color image capture
     
6. Zeiss AxioImager M1 fluorescence microscope for quantitative fluorescence.
   
   1. • Bright field, Dark field, Phase, DIC.
      • Epifluorescence illumination
      • High resolution digital CCD camera (Photometrics Quantix) for 12-bit image capture
        
7. Zeiss AxioObserver inverted fluorescence microscope for live-cell imaging

Bright field, Dark field, Phase, DIC, PlasDIC for plastic plates

1. 1. • Epifluorescence illumination
      • High resolution digital CCD camera (Hamamatsu EMCCD 9100-13) for 16-bit image capture
      • iVision for scripting long-term experiments
2. Zeiss Axiovert for plates
   • Bright field, phase, DIC.
   • Epifluorescence
   • Computer color image capture using Optronics 750 color CCD camera
     
     
3. Zeiss SV11 stereo microscope
   
4. Zeiss Lumar Epifluorescence stereo microscope for low magnification fluorescence imaging
5. Ruzin offers a semester course "Techniques in Light Microscopy".

• Leica LMD UV laser microdissection microscope.

1. Low light gray scale fluorescence detection using a Photometrics Quantix high-resolution CCD digital camera
   
   
2. Automated ratio imaging using iVision
   

1. Color digital image capture
2. Color printing: Photo quality Epson Color Stylus Pro 4000, Tektronix 740 color laser printer
3. Image manipulation and enhancement using Photoshop, iVision, and Bitplane Imaris.
4. Deconvolution of digital image series using SVI Huygens and API SoftWoRx.
5. Morphometrics, particle counting, particle tracking, numeric analysis, Fourier transforms (Imaris)
6. AIT data archiving

Revised Wed, Sep 3, 2008