Technologies available in the
BIOLOGICAL IMAGING FACILITY
UC Berkeley

1. Microtechnique
  1. Paraffin sectioning for general light microscopy and in situ hybridization using Microwaves. We use the Microwave Research and Applications, Inc BP-111-RS tissue processor. Here are some samples.
  2. Plastic (methacrylate) sectioning for general light microscopy, immunolocalization and in situ hybridization.
  3. Cryomicrotomy for immunolocalization and in situ hybridization.
2. Light microscopy
  1. Zeiss LSM 710 confocal laser scanning microscope.
  2. Zeiss LSM 510 Meta UV/Vis confocal laser scanning microscope.
  3. Applied Precision DeltaVision Elite deconvolution microscope.
  4. Bitplane Imaris ("malificent.berkeley.edu") for 3D imaging
  5. SVI HuygensPro for deconvolution ("huygens.berkeley.edu")
  6. iVision for 2D image manipulation and processing
  7. Zeiss AxioImager M2:
    1. Bright field, Dark field, Phase, DIC.
    2. Epifluorescence illumination
    3. High resolution quantitative digital CCD camera (QImaging iClick) for 12-bit image capture
  8. Zeiss AxioImager M1 fluorescence microscope for quantitative fluorescence.
    1. Bright field, Dark field, Phase, DIC.
    2. Epifluorescence illumination
    3. High resolution digital CCD camera (Hamamatsu Orca 03) for 12-bit image capture
  9. Zeiss AxioObserver Z1 inverted fluorescence microscope for live-cell imaging for Bright field, Dark field, Phase, DIC, PlasDIC for plastic plates, Epifluorescence illumination
    1. High resolution digital CCD camera (Hamamatsu EMCCD 9100-13) for 16-bit image capture'
    2. iVision for scripting long-term experiments
  10. Zeiss Axiovert for plates
    1. Bright field, phase, DIC.
    2. Epifluorescence
  11. Zeiss SV11 stereo microscope
  12. Zeiss Stereo Lumar Epifluorescence stereo microscope for low magnification fluorescence imagin
  13. Ruzin offers a semester course "Techniques in Light Microscopy".

  • 3. Low Light Fluorescence for Live-Cell imaging (AxioObserver Z1)

    1. Low light gray scale fluorescence detection using a Hamamatsu EMCCD 9100-13 low-light CCD digital camera
    2. Automated timelapse fluorescence imaging using iVision
    4. Computer image processing and analysis
    1. Color digital image capture
    2. Color printing
    3. Image manipulation and enhancement using Photoshop, iVision, and Bitplane Imaris.
    4. Deconvolution of digital image series using SVI Huygens.
    5. Morphometrics, particle counting, particle tracking, numeric analysis, Fourier transforms (Imaris)
    6. Data backup and archiving

     

    Revised Sun, Dec 2, 2012