Plant biologists may use the IVIS for individual experiments. In this case the User does not need to pass the NAF/LKS Safety trainings, nor have individual Card Key access. However, there are certain restrictions:
For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal. See the Calipers reference library for more information.
The instrument is located on the first floor of LKS. The instrument is suitable for animals and certain plant specimens.
No Invertebrates are permitted