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IVIS Spectrum In Vivo imager
Procedure for IVIS users
Animal Imaging
Imaging Other organisms (including plates)

Plant biologists may use the IVIS for individual experiments. In this case the User does not need to pass the NAF/LKS Safety trainings, nor have individual Card Key access. However, there are certain restrictions:

The IVIS Spectrum is an optical imager used to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. Filters and spectral un-mixing algorithms let you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using a Digital Mouse Atlas.

For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal. See the Calipers reference library for more information.

The instrument is located on the first floor of LKS. The instrument is suitable for animals and certain plant specimens.

No Invertebrates are permitted

Reserve the IVIS
IVIS schematic diagram and Ex/Em possibilities for fluorescence.
Up to five live mice may be scanned at one time.
Modified 4/4/14