Steven Ruzin Ph.D.
CNR Biological Imaging Facility
381 Koshland Hall

The course will be a detailed overview of the practice of light microscopy as applied to scientific investigation. The emphasis of the course will be on the use of the light microscope by biological scientists, however students of other disciplines are welcome. We will cover optical microscope theory, microscope components and mechanics, and all modern optical techniques from Brightfield through Deconvolution microscopy, Structured Illumination, and others. Instruction will consist of lectures, demonstrations, and practice using the light microscopes in the CNR Biological Imaging Facility and the GPBB Teaching facility.

Laboratory: The laboratory is in 209 GPBB, and will consist of one session per week. Each student is responsible for one laboratory session per week. Lab times are:

Section 101 (CCN 70528): Thursday 2–5PM, 209 GPB
Section 102 (CCN 70531): Friday 2–5PM, 209 GPB

Grading: There will be a Midterm written exam and lab practical, plus a written Final Exam. For labs, students will be required to complete at least one worksheet for each of the labs. Worksheet is due at the end of that lab. In addition, a project is required for the class. It consists of a written paper. A description of the project will be given toward the middle of the semester.

Syllabus

Office hour: Wed 4-5PM, 381 Koshland Hall