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Plant Microtechnique
S. Ruzin
1999

Errata

Chapter 6, Sectioning For Silane formulations replace 100µm with 100µl

Graphic showing preparation of glass knife from microscope slides has been redrawn to a more representative view.

Chapter 7 Staining. Johansen's Safranin and Fast Green. The concentration of Fast Green is 0.05% not 0.15%. However, Johansen required a saturates FG solution, so formulations containing the higher amount are just as effective. They only waste FG.
Chapter 12 Common cytochemical fluorescent dyes Change the solvent concentration of the following
NBD Ceramide, R123, DIOC7 to abs DMSO.

Calcofluor White M2R should be used at a working concentration of 0.1mg/ml

Chapter 14; In situ Hybridization, Table: Reagents for immunological detection. For "Color substrates" add:

NBT solution (75mg/ml)
X-phosphate (50mg/ml)

Chapter 14. In situ hybridization Diagram of plasmid is drawn incorrectly.