Protocols |
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| The major problem with microwave processing is penetration of the various solutions under the extremely short processing times when using microwave procedures. In our hands, dense animal tissue (e.g., liver) responded poorly when subjected to multiple steps of 15 minute fixation and infiltration. This "Standard Protocol" was inadequate in allowing penetration and infiltration of large or dense specimens. Thus, we developed the "Extended Mini" protocol, which consists of fewer, but longer, penetration steps.
Here are examples using the MRA microwave tissue processing oven: |
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| Extended Mini Protocol | ||||||||||||||||
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Click on the thumbnail to see a larger image
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Mouse kidney 20x
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Mouse kidney 40x
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Mouse liver 40x
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| Standard Protocol | ||||||||||||||||
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Olive leaf 20x
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Olive leaf 40x
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Coleus Embryo Sac 40x
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Coleus stem 40x
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