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Protocols

The major problem with microwave processing is penetration of the various solutions under the extremely short processing times when using microwave procedures. In our hands, dense animal tissue (e.g., liver) responded poorly when subjected to multiple steps of 15 minute fixation and infiltration. This "Standard Protocol" was inadequate in allowing penetration and infiltration of large or dense specimens. Thus, we developed the "Extended Mini" protocol, which consists of fewer, but longer, penetration steps.

Here are examples using the MRA microwave tissue processing oven:

Extended Mini Protocol
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Mouse kidney 20x
Mouse kidney 40x
Mouse liver 40x

Standard Protocol
Olive leaf 20x
Olive leaf 40x
Coleus Embryo Sac 40x
Coleus stem 40x

8/10/05